Figure 6.
Identification of genes regulated by CBX8 and BMI1. (A) A Venn diagram showing the overlap between genes found to be repressed >1.2-fold by microarray expression profile analysis and 3366 genes identified by ChIP on a chip analysis (Bracken et al, 2006) to be positive for CBX8 binding or the H3K27me3 mark. (B) Direct ChIP in TIG3-T cells using BMI1, CBX8 and H3K27me3 antibodies for the genes listed in Table I. (C) ChIP using anti-CBX8 in TIG3-T treated with RNAi targeting BMI1 (pRS-BMI1) or CBX8 (pRS-CBX8). (D) RT–QPCR showing the relative mRNA levels of the selected genes from Table I in TIG3-T after RNAi-mediated downregulation of CBX8.