Figure 6.
MTHFS-mediated catalysis of 5-formylTHHF. The MTHFS-catalyzed conversion of 5-formylTHHF to methenylTHHF was investigated by monitoring the increase in absorbance at λmax=320nm as described in Materials and Methods. (■) Y151A-MTHFS, (♦) Y152A-MTHFS, (▲) wild-type MTHFS, (●) control (no enzyme).