FIG. 5.
Induction of TRAIL mRNA and protein in response to RSV infection. (A) Production of TRAIL mRNA in RSV-, UV-RSV-, or mock-infected A549 cells, NHBE, and SAEC. Total intracellular RNA was harvested at the indicated times postinfection, and the amount of TRAIL mRNA measured by an RPA is shown as a percentage of the GAPDH mRNA measured in the same gel lane. (B) Expression of transmembrane TRAIL protein at the surface of RSV- or mock-infected A549 cells measured by flow cytometry at the indicated times postinfection. The percentage of TRAIL-positive cells is expressed as the mean ± standard error based on three samples per group. Results obtained with an isotype control antibody were similar to those obtained with mock-infected control cells (not shown).