Table 2.
Genetic Analysis of Offspring Obtained from RelA/TNFR-1 Double Heterozygous Matings
| Genotype | Expected number | Actual number | Expected percentage | Actual percentage |
|---|---|---|---|---|
| RelA+/+ | 6.25 | 9 | 7.2 | 7.82 |
| TNFR-1+/+ | ||||
| RelA+/− | 12.5 | 14 | 14.4 | 12.17 |
| TNFR-1+/+ | ||||
| RelA+/+ | 12.5 | 19 | 14.4 | 16.52 |
| TNFR-1+/− | ||||
| RelA+/− | 25.0 | 33 | 28.8 | 28.70 |
| TNFR-1+/− | ||||
| RelA+/+ | 6.25 | 11 | 7.2 | 9.57 |
| TNFR-1−/− | ||||
| RelA+/− | 12.5 | 22 | 14.4 | 19.13 |
| TNFR-1−/− | ||||
| RelA−/− | 6.25 | 0 | 7.2 | 0 |
| TNFR-1+/+ | ||||
| RelA−/− | 12.5 | 0 | 14.4 | 0 |
| TNFR-1+/− | ||||
| RelA−/− | 6.25 | 7 | 7.2 | 6.09 |
| TNFR-1−/− |
Genotyping was performed by PCR analysis of tail DNA using primers diagnostic for both WT and mutant alleles.