Table 5.
Comparison of computational quarter-site coupling probability and experimental p53–DNA affinity
| Group | P53REs | Q1234 | T13 | T24 | H14 | H23 | Kd (nM)a |
|---|---|---|---|---|---|---|---|
| Very strong binding | 14-3-3 σ s1 | 13 | 13.4 | 20.8 | 44.7 | 8.1 | 9.9 ± 2.1 |
| 14-3-3 σ s2 | 16.1 | 16.7 | 23.6 | 30.5 | 13.1 | 10 ± 2.1 | |
| GADD45 | 18.1 | 21.7 | 20.1 | 24.5 | 15.4 | 7.7 ± 1.2 | |
| Maspin | 18.9 | 18.4 | 22.2 | 22.4 | 18.1 | 9.2 ± 1.7 | |
| MDM2 | 13.7 | 21.8 | 16.5 | 33.6 | 14.4 | 12.3 ± 2.9 | |
| NOXA | 16.3 | 18.8 | 19.9 | 31.3 | 13.8 | 8.6 ± 1.6 | |
| P21-5′ site | 17.7 | 21.2 | 24.5 | 20 | 16.6 | 4.9 ± 0.6 | |
| P21-3′ site | 16.3 | 24.3 | 15.8 | 30.9 | 12.8 | 12 ± 7.0 | |
| P53AIP1 | 16.5 | 17.8 | 27.1 | 21.5 | 17 | 11.0 ± 1.8 | |
| P53R2 | 15.9 | 16 | 21.6 | 33.1 | 13.5 | 5.7 ± 0.8 | |
| PCNA | 17.1 | 26.2 | 20.2 | 21.5 | 15.1 | 6.6 ± 1.4 | |
| PIDD | 19 | 17.2 | 23.9 | 19.8 | 20.1 | 14.7 ± 5.0 | |
| PUMA-BS2 | 14.4 | 14.7 | 24.5 | 33.3 | 13.1 | 7.1 ± 1.8 | |
| Cyclin G | 14.5 | 15.4 | 20.8 | 37.3 | 12 | 7.8 ± 1.6 | |
| Group average | 16.3 | 18.8 | 21.6 | 28.9 | 14.5 | 9.1 | |
| Strong binding | KAI 1 | 18.9 | 19 | 22 | 24.7 | 15.4 | 33 ± 10.9 |
| Group average | 18.9 | 19 | 22 | 24.7 | 15.4 | 33 ± 10.9 | |
| Weak binding | Bax | 18.9 | 18.1 | 22.1 | 19 | 21.8 | 73 ± 33 |
| IGF-BP3 box A | 18.6 | 18.8 | 21.8 | 23 | 17.7 | 81 ± 25 | |
| P53DINP1 | 17 | 26.2 | 18.1 | 25.6 | 13 | 98 ± 23 | |
| Group average | 18.2 | 21 | 20.7 | 22.5 | 17.5 | 84 | |
| Very weak binding | P2XM | 18.7 | 21.3 | 19 | 24.3 | 16.7 | 221 ± 41 |
| PUMA-BS1 | 18.2 | 15.9 | 26.4 | 12.6 | 26.9 | 260 ± 50 | |
| Group average | 18.5 | 18.6 | 22.7 | 18.5 | 21.8 | 241 |
a Reference (13).