Table 1.
Influence of peptides on the chaperone activity of Hsp90 fragments
| Peptide | Sequence | Turbidity
|
|
|---|---|---|---|
| N210 | 262C | ||
| No peptide | — | 30 | 30 |
| HD 25 | SGFTFND | 35 | n.d. |
| HD 86 | LRAEDMA | 39 | n.d. |
| HD 131 | GPSVFPL | 53 | 58 |
| HD 131 L | GPSVFPLAPS SKC | 95 | 56 |
| HD 132 | PSVFPLA | 38 | 60 |
| HD 133 | SVFPLAPS | 55 | 58 |
| HD 131 + HD 133 | GPSVFPL + SVFPLAPS | 51 | n.d. |
| HD 167 | WNSGALT | 34 | n.d. |
| GR 1 | AKAILGLRNL HLDDQMTLL | 92 | 60 |
| GR 3 | AKAILGLRN | 76 | 55 |
| GR 4 | LRNLHLDDQ | 65 | 62 |
| GR 5 | LHLDDQMTLL | 46 | 54 |
| GR 3 + GR 5 | AKAILGLRN + LHLDDQMTLL | 80 | n.d. |
| VSV 8 | RGYVYQGL | 71 | 53 |
Aggregation of insulin was measured as described above. The numbers represent the normalized turbidity in arbitrary units determined 35 min after reduction of insulin.
n.d., not determined.