Table 1.
Occurrences of proteins directly crosslinked to keratins
| CE experiment number | Number of peptides | Cystatin α | Elafin | Envoplakin | Involucrin | Loricrin | SPRs 1 and 3 | |
|---|---|---|---|---|---|---|---|---|
| Keratin 1 | ||||||||
| 1 | 6 | 6 (70) | ||||||
| 2 | 8 | 1 (15) | 3 (50) | 1 (40) | 3 (250) | |||
| 3 | 31 | 1 (10) | 1 (10) | 19 (240) | 1 (50) | 4 (600) | 5 (190) | |
| 4 | 7 | 1 (10) | 4 (80) | 1 (110) | 1 (25) | |||
| 5 | 19 | 1 (15) | 1 (15) | 2 (25) | 9 (140) | 4 (220) | 2 (40) | |
| Keratin 2e | ||||||||
| 1 | 1 | 1 (5) | ||||||
| 2 | 4 | 2 (20) | 1 (20) | 1 (50) | ||||
| 3 | 3 | 1 (5) | 1 (10) | 1 (50) | ||||
| 4 | 1 | 1 (10) | ||||||
| 5 | 2 | 2 (40) | ||||||
| Keratin 5 (or 6) | ||||||||
| 1 | 0 | |||||||
| 2 | 12 | 7 (160) | 4 (60) | 1 (10) | ||||
| 3 | 33 | 1 (10) | 2 (20) | 22,1 (340) | 1 (10) | 5 (60) | 1 (10) | |
| 4 | 67 | 1 (5) | 1 (10) | 34,3 (650) | 21,3 (270) | 1 (5) | 2 (10) | 1 (10) |
| 5 | 55 | 2 (20) | 3 (50) | 18 (250) | 14,3 (240) | 8,1 (110) | 5 (40) | 1 (5) |
| Keratin 10 | ||||||||
| 1 | 1 | 1 (10) | ||||||
| 2 | 2 | 2 (20) | ||||||
| 3 | 1 | 1 (20) | ||||||
| 4 | 0 | |||||||
| 5 | 3 | 1 (10) | 2 (30) | |||||
The numbers in parenthesis are yields in pmol. CE experiment numbers are: 1, foreskin stratum corneum (14); 2, saponified foreskin stratum corneum (15); 3, “immature” foreskin epidermis (15); 4, 3-day NHEK cultures (P.M.S. and L.N.M., unpublished data); 5, 7-day NHEK cultures (P.M.S. and L.N.M., unpublished data). In most cases, the short sequences of the keratin branch did not permit distinction between the keratin 5 or 6 chains. The second number listed defines known keratin 6 sequences; e.g., 22,1 (340) means that of a total of 23 peptides (totaling 340 pmol), 22 involved the keratin 5 or 6 chains and 1 was confirmed from keratin 6.