Fig. 2.
Defects of the cerebellum in Lhx1/Lhx5 double mutants (Lhx1fx1/LacZ; Nes-Cre+; Lhx5−/−). The cerebellum of mutant embryos (B, D, F, H, J, and L), as compared with that of control embryos (A, C, E, G, I, and K), was analyzed by hematoxylin and eosin staining (A–D) and immunostaining of calbindin (E–H) and Pax2 (I–L) at E14.5 (A, B, E, F, I, and J) and E18.5 (C, D, G, H, K, and L). Arrows in A and B point at the cells of the developing deep cerebellar nuclei. Arrowheads in C, E, and G point out the developing Purkinje cells in the cerebellum. The arrowhead in H shows residual calbindin-positive cells present in the Lhx1/Lhx5 double mutants. (Scale bar: 100 μm.)