Figure 2.
Overexpression of TCP20 suppresses the growth defect of a tor2ts mutation but not of a tor1 tor2ts double mutation. Wild-type TOR2 (JK9-3da), tor2ts (SH121), and tor1 tor2ts cells (SH221) carrying an empty vector or pTCP20 (pAS27) were streaked on YPD and incubated at 30°C or 37°C. tor2ts and tor1 tor2ts cells carrying an empty vector are not viable at 37°C.