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. 1998 Mar 3;95(5):2592–2596. doi: 10.1073/pnas.95.5.2592

Table 1.

Proliferation assays with heterozygous and homozygous N-CAM knockout astrocytes

Reagent Concentration Heterozygous N-CAM knockout cells (−/+)
Homozygous N-CAM knockout cells (−/−)
[3H]thymidine incorporation, % of control [3H]thymidine incorporation, % of control
N-CAM 5 μg/ml 30  ±  1 101  ±  8
N-CAM 2 μg/ml 57  ±  7 115  ±  2
Ig III 3 μg/ml 29  ±  4 94  ±  5
Ig III 1 μg/ml 33  ±  2 106  ±  3

Astrocytes were obtained from transgenic mice that were heterozygous or homozygous for the disrupted N-CAM allele. N-CAM mRNA and protein was not detected in homozygous astrocytes (13). The amount of [3H]thymidine incorporation is presented as the percent incorporated compared to astrocytes treated with PBS alone. Addition of N-CAM or Ig III recombinant protein decreased the amount of [3H]thymidine incorporation in heterozygous but not homozygous astrocytes. Data are presented as mean ± SD (n = 4 for each condition).