Figure 2.
PN-mediated cell death is prevented by PN decomposition catalysts. Cells were treated as described in Materials and Methods. (A) Titration of cellular injury with increasing pulses of PN. (B) FeTMPS protected RAW 264.7 cells from a 200-μM PN challenge (closed triangles). In contrast, the addition of TMPS did not protect the cells (open triangles). The solid line indicates the percentage of viability of PN-treated cells in the absence of compound. (C) Western blot for nitrotyrosine content of released cellular proteins after PN (200 μM) treatment in the presence of 100 μM FeTMPS (lane 2) or TMPS (lane 1). Cellular viability is depicted in the bar graph. Each point represents the mean of four individual wells ± SEM. Data are representative of at least three independent experiments.