Fig. 2.
Effects on inflammation and wound healing. (A) Cytokine production in LPS-stimulated peritoneal macrophages. Results are shown as the mean ± SE (n = 10) of triplicate experiments. *, P < 0.05. (B) Spontaneous skin lesions in PI3KγCX/CX mice. (Left) Back skin lesion area (×10 magnification). (Right) Section of the lesion showing accumulation of neutrophils (arrows). (Scale bar: 100 μm.) (Inset) Their high power magnification. (Inset scale bar: 50 μm.) (C) Full-thickness cutaneous wounds of control (PI3Kγ+/+) and PI3KγCX/CX mice sectioned 10 days after wounding and stained with hematoxylin/eosin (filled arrowhead, eschar; open arrowhead, granulation tissue). (Scale bar: 1 mm.) (Insets) High-power magnification of dermis with infiltration of inflammatory cells in the mutant sample. (Inset scale bar: 100 μm.) (D) Analysis of leukocyte apoptosis in sections as in B. Leukocytes are labeled with CD18 (green) and apoptosis with TUNEL (red). (Magnification: ×63.) The number of CD18+/TUNEL+ cells was counted in four random high-power fields (×40) within the region adjacent to the wound for each animal (n = 7). *, P < 0.05.