Table 2.
Loss of function of M04F3.1, Y71F9AL.18 and R06C7.7 results in frequent insertion/deletion mutations
| RNAi against gene
|
Frameshift mutationsa
|
Mutation type (del =deletions; ins = insertions)
|
Mutated sequence: unc-93 mutational target
|
|---|---|---|---|
| M04F3.1 | 10/12 | del. nt 21 | TGGCTTTTTCCAGATactcttctatgatccctgcttCAATATTCTGTGGAA |
| del. nt 27 | ATATTTGACGCGCaatttcatatctcctctcaggtttggcCACTAAATGCAGC | ||
| del. A | TATGTAGGTATGTAGGaTATGTAGGTATGTA | ||
| G→ACT | TCTACATTTTGCTA(G→ACT)TTGACAACTTT | ||
| TA→ACT | ACTAACCTTTGTA(TA→ACT)AACTCCAACT | ||
| G→AT | GACCAATTTATTAC(G→AT)TGGAAGTCTTC | ||
| ins. A | AAAATACTTTGGGCTCACTCGAAAAACTCT | ||
| ins. A | AACTGATTTTTTTGATATTTTAATTATTTTC | ||
| ins. T | CATGCATTCGGTAGATTGATGAAGATAATG | ||
| ins. T | AAACATCGGTGGCCGTAGTTTTCCAATTTTC | ||
| Y71F9AL.18 | 6/9 | del. nt 1184→ins. nt 523 | AAATTTGAA(del. nt 2147–3331→ins. nt 4358–4880)GGCCACCAC |
| del. AAAA | TTTGGAGCGATTCCAaaaaAGAAGGATGATAG | ||
| TA→GAGCCCAAA | CGAGGAGCCCAAA(TA→GAGCCCAAA)TTTTCCTTTCGAG | ||
| ins. AC | TTCAGTGGGAAGTTCACGAAATGATCGTCGT | ||
| ins. A | TTTCTCTTCCTGTTCAACTGCATTTAACGGAC | ||
| ins. A | AAGAGATTCCCAAAAATCGATATTTAGTGAA | ||
| R06C7.7 | 7/10 | del. CATCAT | TATCACTTGCCATATcatcatTATTTGTTCCATC |
| del. GTCATT | TATTTTCTATACATTgtcattAATTTGAGGCCGA | ||
| del. AAAC | AATTGGTCACAATGCaaacTCTCATGCATTCG | ||
| del. TCT | TTTTTCCAGATACTCTtctATGATCCCTGCTTC | ||
| del. N10→ins. GT | AAAACTTCGATT(catcttttgc→GT)AAAAATTTG | ||
| del. N10→ins. T16 | TCATATCTCC(tctcaggttt→TTTTTTTTTTTTTTTT)GGCCACTAAA | ||
| ins. T | CTACATACCTACATATTCTACAAACCTACAT |
a
The remainder are single base pair substitutions (not depicted).