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. 2005 Jan 3;201(1):27–33. doi: 10.1084/jem.20040989

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — The conserved region I likely contains the proteolytic cleavage site. (A) The epitopes recognized by the NH₂-terminal antiserum were determined by ELISA by testing its reactivity to overlapping peptides encompassing the NH₂-terminal third of CSP. Pep 1, GYGQNKSIQAQRNLNE; Pep 2, RNLNELCYNEGNDNKL; Pep 3, NDNKLYHVLNSKNGKI; Pep 4, KNGKIYIRNTVNRLLA; Pep 5, NRLLADAPEGKKNEKK; Pep 6, KNEKKNKIERNNKLK; and N-term, full-length NH₂-terminal peptide. (B–D) Proteolytic cleavage of hybrid CSP. (B) Structure of CSP from Pf/Pb sporozoites. The portion of P. berghei CSP that has been replaced with the orthologous sequence of P. falciparum CSP is shown in orange and includes region I, which is shown in black. (C) Western blot of Pf/Pb sporozoites probed with mAb 2A10, directed against the P. falciparum CSP repeat region. (D) Pf/Pb sporozoites were metabolically labeled and kept on ice (lane 1) or chased with cold medium for 4 h, in the absence or presence of E-64. Samples were processed as outlined in Fig. 1.