Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1987 Nov;56(5):633–636. doi: 10.1038/bjc.1987.257

Survival of large bowel carcinoma patients with different DNA ploidy.

T O Rognum 1, E Thorud 1, E Lund 1
PMCID: PMC2001905  PMID: 3426928

Abstract

One hundred patients operated for large bowel carcinoma were divided into a distinct aneuploid group of 63, and a near diploid one of 37. Flow cytometry was used for determination of the DNA ploidy pattern. All tumours in the aneuploid group contained one or more aneuploid cell populations. All patients were followed clinically from 3.5 to 7.8 years. The corrected 5 year survival was 64% and 49% for patients with near diploid and aneuploid tumours, respectively (not significant). Significant differences in corrected survival time were not observed for Dukes' stages A, B, and C patients pooled, nor for Dukes' stage D patients. However, for Dukes' stage C patients alone, there was a tendency (P = 0.10) for patients with near diploid tumours to show a better survival. A highly significant predominance of aneuploid tumours was seen in males, in contrast to an equal distribution of aneuploid and near diploid tumours in females. A slight predominance of aneuploid tumours in the left colon and rectum was seen. Both these findings indicate the influence of environmental factors (hormonal, anatomical, phenotypical) on the development of tumours with a particular DNA ploidy pattern.

Full text

PDF
633

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe S., Makimura S., Itabashi K., Nagai T., Tsuneta Y., Kawakami Y. Prognostic significance of nuclear DNA content in small cell carcinoma of the lung. Cancer. 1985 Oct 15;56(8):2025–2030. doi: 10.1002/1097-0142(19851015)56:8<2025::aid-cncr2820560823>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  2. Armitage N. C., Robins R. A., Evans D. F., Turner D. R., Baldwin R. W., Hardcastle J. D. The influence of tumour cell DNA abnormalities on survival in colorectal cancer. Br J Surg. 1985 Oct;72(10):828–830. doi: 10.1002/bjs.1800721018. [DOI] [PubMed] [Google Scholar]
  3. Atkin N. B., Kay R. Prognostic significance of modal DNA value and other factors in malignant tumours, based on 1465 cases. Br J Cancer. 1979 Aug;40(2):210–221. doi: 10.1038/bjc.1979.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Atkin N. B. Prognostic significance of ploidy level in human tumors. I. Carcinoma of the uterus. J Natl Cancer Inst. 1976 May;56(5):909–910. doi: 10.1093/jnci/56.5.909. [DOI] [PubMed] [Google Scholar]
  5. Finan P. J., Quirke P., Dixon M. F., Dyson J. E., Giles G. R., Bird C. C. Is DNA aneuploidy a good prognostic indicator in patients with advanced colorectal cancer? Br J Cancer. 1986 Aug;54(2):327–330. doi: 10.1038/bjc.1986.180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedlander M. L., Hedley D. W., Taylor I. W. Clinical and biological significance of aneuploidy in human tumours. J Clin Pathol. 1984 Sep;37(9):961–974. doi: 10.1136/jcp.37.9.961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kokal W., Sheibani K., Terz J., Harada J. R. Tumor DNA content in the prognosis of colorectal carcinoma. JAMA. 1986 Jun 13;255(22):3123–3127. [PubMed] [Google Scholar]
  8. Laerum O. D., Farsund T. Clinical application of flow cytometry: a review. Cytometry. 1981 Jul;2(1):1–13. doi: 10.1002/cyto.990020102. [DOI] [PubMed] [Google Scholar]
  9. Mantel N. Evaluation of survival data and two new rank order statistics arising in its consideration. Cancer Chemother Rep. 1966 Mar;50(3):163–170. [PubMed] [Google Scholar]
  10. Melamed M. R., Enker W. E., Banner P., Janov A. J., Kessler G., Darzynkiewicz Z. Flow cytometry of colorectal carcinoma with three-year follow-up. Dis Colon Rectum. 1986 Mar;29(3):184–186. doi: 10.1007/BF02555020. [DOI] [PubMed] [Google Scholar]
  11. Perez D. J., Taylor I. W., Milthorpe B. K., McGovern V. J., Tattersall M. H. Identification and quantitation of tumour cells in cell suspensions: a comparison of cytology and flow cytometry. Br J Cancer. 1981 Apr;43(4):526–531. doi: 10.1038/bjc.1981.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Quirke P., Dixon M. F., Clayden A. D., Durdey P., Dyson J. E., Williams N. S., Bird C. C. Prognostic significance of DNA aneuploidy and cell proliferation in rectal adenocarcinomas. J Pathol. 1987 Apr;151(4):285–291. doi: 10.1002/path.1711510408. [DOI] [PubMed] [Google Scholar]
  13. Quirke P., Dyson J. E., Dixon M. F., Bird C. C., Joslin C. A. Heterogeneity of colorectal adenocarcinomas evaluated by flow cytometry and histopathology. Br J Cancer. 1985 Jan;51(1):99–106. doi: 10.1038/bjc.1985.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rognum T. O. A new approach in carcinoembryonic antigen-guided follow-up of large-bowel carcinoma patients. Scand J Gastroenterol. 1986 Aug;21(6):641–649. doi: 10.3109/00365528609011095. [DOI] [PubMed] [Google Scholar]
  15. Rognum T. O., Brandtzaeg P., Thorud E. Is heterogeneous expression of HLA-dr antigens and CEA along with DNA-profile variations evidence of phenotypic instability and clonal proliferation in human large bowel carcinomas? Br J Cancer. 1983 Oct;48(4):543–551. doi: 10.1038/bjc.1983.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rognum T. O., Heier H. E., Orjasaeter H., Thorud E., Brandtzaeg P. Comparison of two CEA assays in primary and recurrent large bowel carcinoma with different DNA ploidy pattern. Eur J Cancer Clin Oncol. 1986 Oct;22(10):1165–1169. doi: 10.1016/0277-5379(86)90317-2. [DOI] [PubMed] [Google Scholar]
  17. Rognum T. O., Thorud E., Brandtzaeg P., Orjasaeter H., Heier H. E., Dahl E., Hognestad J. Plasma CEA in large bowel carcinoma: which patients should be followed by regular postoperative measurements? Preliminary follow-up results in 100 patients with different tumor DNA-ploidy patterns. Cancer Detect Prev. 1987;10(5-6):347–352. [PubMed] [Google Scholar]
  18. Rognum T. O., Thorud E., Elgjo K., Brandtzaeg P., Orjasaeter H., Nygaard K. Large-bowel carcinomas with different ploidy, related to secretory component, IgA, and CEA in epithelium and plasma. Br J Cancer. 1982 Jun;45(6):921–934. doi: 10.1038/bjc.1982.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thorud E., Fosså S. D., Vaage S., Kaalhus O., Knudsen O. S., Børmer O., Shoaib M. C. Primary breast cancer. Flow cytometric DNA pattern in relation to clinical and histopathologic characteristics. Cancer. 1986 Feb 15;57(4):808–811. doi: 10.1002/1097-0142(19860215)57:4<808::aid-cncr2820570421>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
  20. Turnbull R. B., Jr, Kyle K., Watson F. R., Spratt J. Cancer of the colon: the influence of the no-touch isolation technic on survival rates. Ann Surg. 1967 Sep;166(3):420–427. doi: 10.1097/00000658-196709000-00010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Valet G., Rüssmann L., Wirsching R. Automated flow-cytometric identification of colo-rectal tumour cells by simultaneous DNA, CEA-antibody and cell volume measurements. J Clin Chem Clin Biochem. 1984 Dec;22(12):935–942. [PubMed] [Google Scholar]
  22. Volm M., Brüggemann A., Günther M., Kleine W., Pfleiderer A., Vogt-Schaden M. Prognostic relevance of ploidy, proliferation, and resistance-predictive tests in ovarian carcinoma. Cancer Res. 1985 Oct;45(10):5180–5185. [PubMed] [Google Scholar]
  23. Volm M., Drings P., Mattern J., Sonka J., Vogt-Moykopf I., Wayss K. Prognostic significance of DNA patterns and resistance-predictive tests in non-small cell lung carcinoma. Cancer. 1985 Sep 15;56(6):1396–1403. doi: 10.1002/1097-0142(19850915)56:6<1396::aid-cncr2820560630>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  24. Wolley R. C., Schreiber K., Koss L. G., Karas M., Sherman A. DNA distribution in human colon carcinomas and its relationship to clinical behavior. J Natl Cancer Inst. 1982 Jul;69(1):15–22. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES