Figure 5.
CyPPA does not inhibit NS309-induced activation of hSK1. (a) hSK1 current at −75 mV as a function of time. The current was measured from an inside-out patch obtained from a HEK293 cell stably expressing hSK1. The patch was exposed to a bath/intracellular [Ca2+] of 0.01, 0.2 or 10 μM as indicated. NS309 (10 μM) was added at a [Ca2+] of 0.2 μM either in the absence or presence of 10 μM CyPPA. (b) Increase in hSK1 current induced by NS309 (0.1 or 10 μM) in the absence or presence of 10 μM CyPPA; n=6–13.