Figure 4.
(A–C) Relative mRNA levels in hypothalamus of IL-1β-deficient mice (−/−) and wild-type mice (+/+), after injection of LPS (5 mg per kg of body weight, i.p.) [LPS]; rrIL-1α (10 μg per kg of body weight, i.p.) [IL-1α]; rrIL-1β (10 μg per kg of body weight, i.p.) [IL-1β]; or saline [contr]. Ratios of respective mRNA and β-actin mRNA are presented. ∗∗, P < 0.01, and ∗, P < 0.05, vs. control +/+; and ¤, P < 0.05, vs. IL-1α +/+. (A) IL-1α mRNA levels. Control +/+, n = 7; control −/−, n = 7; LPS +/+, n = 3; LPS −/−, n = 3; IL-1α +/+, n = 3; IL-1α −/−, n = 3; IL-1β +/+, n = 5; IL-1β −/−, n = 5. (B) IL-6 mRNA levels. Control +/+, (n = 7); control −/−, (n = 7); LPS +/+, n = 3; LPS −/−, n = 3; IL-1α +/+, n = 3; IL-1α −/−, n = 3; IL-1β +/+, n = 5; and IL-1β −/−, n = 5. (C) TNFα mRNA levels. Control +/+, n = 8; control −/−, n = 7; LPS +/+, n = 3; LPS −/−, n = 3; IL-1α +/+, n = 3; IL-1α −/−, n = 3; IL-1β +/+, n = 5; and IL-1β −/−, n = 5.