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JIL-1 is required for P-TEFb recruitment but not DSIF binding after heat shock in JIL-1-null mutants. Detail view of the heat-shock puffs at 87A/C stained with CycT (red) (A,B) and SPT5 (red) (C,D) antibodies from wild-type (A,C) and JIL-1z2 (B,D) polytene chromosomes under heat-shock conditions. Pol IIo ser5 (green) was used as control, and DNA is stained with DAPI (blue) in A–D.
