Abstract
When rat kidney slices were incubated in the presence of horseradish peroxidase, there was an energy-dependent uptake of the protein by the cells of the kidney tubules. The uptake was greatest in the proximal convoluted tubules and in the thick ascending limbs of the loops of Henle; it was abolished by cold, anoxia, 2,4-dinitrophenol, and fluoroacetate, and was more readily depressed by unfavorable metabolic conditions in the proximal convoluted tubules than in the thick ascending limbs. Protein uptake was inhibited when the kidney slices were incubated in electrolyte-free media. In sodium chloride solutions, uptake was reduced as sodium was progressively replaced by choline, and ouabain inhibited uptake in the proximal convoluted tubules, but not in the thick ascending limbs. To a limited extent, lithium could replace sodium in the incubation medium with no depression of peroxidase uptake. These results suggest that a sodium-stimulated, ouabain-sensitive ATPase may be involved in the uptake of protein by cells of the kidney tubule. The intracellular transport of peroxidase in cells of the proximal convoluted tubules was abolished by cold, anoxia, and 2,4-dinitrophenol, but it was not affected by concentrations of ouabain which inhibited the uptake of the protein.
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Selected References
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- BRANDT P. W. A study of the mechanism of pinocytosis. Exp Cell Res. 1958 Oct;15(2):300–313. doi: 10.1016/0014-4827(58)90032-6. [DOI] [PubMed] [Google Scholar]
- CAULFIELD J. B. STUDIES ON FERRITIN UPTAKE BY ISOLATED TUMOR CELLS. Lab Invest. 1963 Oct;12:1018–1025. [PubMed] [Google Scholar]
- CROSS R. J., TAGGART J. V. Renal tubular transport: accumulation of p-aminohippurate by rabbit kidney slices. Am J Physiol. 1950 Apr 1;161(1):181–190. doi: 10.1152/ajplegacy.1950.161.1.181. [DOI] [PubMed] [Google Scholar]
- DE TERRA N., RUSTAD R. C. The dependence of pinocytosis on temperature and aerobic respiration. Exp Cell Res. 1959 Apr;17(1):191–195. doi: 10.1016/0014-4827(59)90166-1. [DOI] [PubMed] [Google Scholar]
- GORDON G. B., KING D. W. Phagocytosis. Am J Pathol. 1960 Sep;37:279–292. [PMC free article] [PubMed] [Google Scholar]
- HOLT S. J., HOBBIGER E. E., PAWAN G. L. Preservation of integrity of rat tissues for cytochemical staining purposes. J Biophys Biochem Cytol. 1960 Apr;7:383–386. doi: 10.1083/jcb.7.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MILLER F. Hemoglobin absorption by the cells of the proximal convoluted tubule in mouse kidney. J Biophys Biochem Cytol. 1960 Dec;8:689–718. doi: 10.1083/jcb.8.3.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RYSER H. J. THE MEASUREMENT OF I131-SERUM ALBUMIN UPTAKE BY TUMOR CELLS IN TISSUE CULTURE. Lab Invest. 1963 Oct;12:1009–1017. [PubMed] [Google Scholar]
- SBARRA A. J., KARNOVSKY M. L. The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. J Biol Chem. 1959 Jun;234(6):1355–1362. [PubMed] [Google Scholar]
- STRAUS W. Cytochemical investigation of phagosomes and related structures in cryostat sections of the kidney and liver of rats after intravenous administration of horseradish peroxidase. Exp Cell Res. 1962 Jun;27:80–94. doi: 10.1016/0014-4827(62)90045-9. [DOI] [PubMed] [Google Scholar]
- WHITTAM R., WILLIS J. S. ION MOVEMENTS AND OXYGEN CONSUMPTION IN KIDNEY CORTEX SLICES. J Physiol. 1963 Aug;168:158–177. doi: 10.1113/jphysiol.1963.sp007184. [DOI] [PMC free article] [PubMed] [Google Scholar]