Abstract
A somatic cell mutant (CR1) of a Chinese hamster ovary cell (CHO-K1) which has previously been shown to be defective in the regulation of cholesterol biosynthesis accumulates more cholesterol than the parental cell line in plasma membranes. Although such an increase in membrane cholesterol should lead to an increase in the order parameter of these membranes, as measured with an electron spin resonance spin probe, the order parameters of mutant and wild-type plasma membranes are identical- -apparently because of an adaptive alteration in membrane phospholipid composition. The phospholipid compositions of mutant and wild-type cell plasma membranes are compared and the mutant is shown to have a threefold higher level of oleic acid and a twofold lower level of phosphatidylethanolamine than the wild type. These results are consistent with model studies which show that these compositional changes lead to lower-order parameters for phospholipid dispersions.
Full Text
The Full Text of this article is available as a PDF (280.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Freter C. E., Ladenson R. C., Silbert D. F. Membrane phospholipid alterations in response to sterol depletion of LM cells. Metabolic studies. J Biol Chem. 1979 Aug 10;254(15):6909–6916. [PubMed] [Google Scholar]
- Haigh W. G., Morris L. J., James A. T. Acetylenic acid biosynthesis inCrepis rubra. Lipids. 1968 Jul;3(4):307–312. doi: 10.1007/BF02530929. [DOI] [PubMed] [Google Scholar]
- Horwitz A. F., Klein M. P., Michaelson D. M., Kohler S. J. Magnetic resonance studies of membrane and model membrane systems. V. Comparisons of aqueous dispersions of pure and mixed phospholipids. Ann N Y Acad Sci. 1973 Dec 31;222:468–488. doi: 10.1111/j.1749-6632.1973.tb15281.x. [DOI] [PubMed] [Google Scholar]
- Hubbell W. L., McConnell H. M. Molecular motion in spin-labeled phospholipids and membranes. J Am Chem Soc. 1971 Jan 27;93(2):314–326. doi: 10.1021/ja00731a005. [DOI] [PubMed] [Google Scholar]
- Kao F. T., Puck T. T. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1275–1281. doi: 10.1073/pnas.60.4.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michaelson D. M., Horwitz A. F., Klein M. P. Head group modulation of membrane fluidity in sonicated phospholipid dispersions. Biochemistry. 1974 Jun 4;13(12):2605–2612. doi: 10.1021/bi00709a021. [DOI] [PubMed] [Google Scholar]
- Nandini-Kishore S. G., Mattox S. M., Martin C. E., Thompson G. A., Jr Membrane changes during growth of Tetrahymena in the presence of ethanol. Biochim Biophys Acta. 1979 Mar 8;551(2):315–327. doi: 10.1016/0005-2736(89)90009-6. [DOI] [PubMed] [Google Scholar]
- Nozawa Y., Kasai R., Sekiya T. Modification of membrane lipids. Phenethyl alcohol-induced alteration of lipid composition in Tetrahymena membranes. Biochim Biophys Acta. 1979 Mar 23;552(1):38–52. doi: 10.1016/0005-2736(79)90244-x. [DOI] [PubMed] [Google Scholar]
- Rintoul D. A., Sklar L. A., Simoni R. D. Membrane lipid modification of chinese hamster ovary cells. Thermal properties of membrane phospholipids. J Biol Chem. 1978 Oct 25;253(20):7447–7452. [PubMed] [Google Scholar]
- Rottem S., Yashouv J., Ne'eman Z., Razin S. Cholesterol in mycoplasma membranes. Composition, ultrastructure and biological properties of membranes from Mycoplasma mycoides var. capri cells adapted to grow with low cholesterol concentrations. Biochim Biophys Acta. 1973 Nov 16;323(4):495–508. doi: 10.1016/0005-2736(73)90158-2. [DOI] [PubMed] [Google Scholar]
- Sinensky M. Defective regulation of cholesterol biosynthesis and plasma membrane fluidity in a Chinese hamster ovary cell mutant. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1247–1249. doi: 10.1073/pnas.75.3.1247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sinensky M. Homeoviscous adaptation--a homeostatic process that regulates the viscosity of membrane lipids in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Feb;71(2):522–525. doi: 10.1073/pnas.71.2.522. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sinensky M. Isolation of a mammalian cell mutant resistant to 25-hydroxy cholesterol. Biochem Biophys Res Commun. 1977 Oct 10;78(3):863–867. doi: 10.1016/0006-291x(77)90502-2. [DOI] [PubMed] [Google Scholar]