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. 2006 Oct 2;203(10):2377–2389. doi: 10.1084/jem.20060845

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — Effect of CO on interactions of TLRs and adaptor molecules. RAW 264.7 cells were treated with 100 ng/ml LPS or 20 μg/ml poly(I:C) in the absence or presence of CO. (A) Interaction between TLR4 and MyD88 or TLR4 and TRIF was analyzed with immunoprecipitation and immunoblotting. Expression of TLR4, MyD88, and TRIF was analyzed by immunoblotting using total cell lysates. (B) Interaction of TLR3 and TRIF was analyzed with immunoprecipitation and immunoblotting.