Figure 8.
The Npl3p import pathway does not overlap with other import pathways. (a) Localization of an SV-40 NLS-GFP reporter was examined in living WT and mtr10 deletion cells and appeared nuclear in both cases. (b) Immunofluorescent localization of Nab2p in the mtr10 deletion strain shows correct nuclear localization. (c) Localization of Npl3p and Nab2p in a kap104:: HIS3 deletion strain at 23°C shows only mislocalization of Nab2p.