Table I.
Anti-TM4 mAbs Inhibit EC Random Motility on Gelatin
| mAb | Cell 1 | Cell 2 | Cell 3 | Cell 4 | Cell 5 | Cell 6 | Average speed | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| mean ± SD | ||||||||||||||
| None | 91.81 | 67.54 | 63.01 | 56.42 | 78.84 | 97.12 | 75.79 ± 16.29 | |||||||
| TEA 1/31 (VE-cadherin) | 100.52 | 64.84 | 69.27 | 59.82 | 66.52 | 58.8 | 69.96 ± 15.4 | |||||||
| TS 2/16 (β1) | 35.39 | 36.91 | 50.85 | 27.01 | 27.7 | 25.94 | 33.97 ± 9.4 | |||||||
| 5A6 (CD81) | 27.59 | 23.92 | 25.94 | 35.51 | 41.49 | 19.94 | 29.06 ± 7.96 | |||||||
| LIA 1/1 (CD151/PETA3) | 54.70 | 40.29 | 47.21 | 36.32 | 35.42 | 31.37 | 40.88 ± 8.6 |
Endothelial cells were plated on gelatin (0.5% in DW) coated plastic dishes and cultured for 24 h before filming starts. Films of cells were generated from stacks of individual digital photographs (frames) taken every 5 min for 5 h in an Axiovert 135 Zeiss videomicroscope using the IP-LAB-SPECTRUM software (Signal Analytics Corporation, Vienna, Virginia) and the distances migrated by individual cells were quantitated using the CELL TRACKING software extension developed by Tim Hutton (Confocal Microscopy and Digital Image Unit, Imperial Cancer Research Fund, UK). Numbers represent average speeds of migration of individual cells over a 5-h period in μm/h. mAb TEA 1/31 (anti–VE-cadherin) has no effect on EC motility and was used as a negative control. Anti β1 mAb TS2/16, which activates β1 integrins, has been used as a control with inhibitory effect on EC motility.