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Increased immunodetection of tau protein in cytoskeletal fraction of sciatic nerve from the NF-H knockout mice. After SDS-PAGE, the protein samples described in Fig. 5 were blotted on membrane and treated with alkaline phosphatase. The membrane was then incubated with the TAU-1 antibody (Boehringer Mannheim Corp., Indianapolis, IN) that recognizes hypophosphorylated tau. The sample from the NF-H −/− yielded an increased signal for a band corresponding to low molecular weight tau.
