Table I.
The Inhibitory Effects of the Radixin–antisense Oligonucleotides Are Reversible
| Antisense treatment | Hours after release from antisense treatment | |||||
|---|---|---|---|---|---|---|
| 0 | 12 | 24 | ||||
| Nontreated | ||||||
| Radixin fluorescence intensity | 125 ± 10 | 128 ± 8 | 132 ± 8 | |||
| Moesin fluorescence intensity | 106 ± 4 | 110 ± 6 | 110 ± 4 | |||
| Growth cone area | 255 ± 35 | 240 ± 28 | ||||
| Mean number actin ribs | 35 ± 5 | 38 ± 6 | ||||
| Radixin–moesin sense-treated | ||||||
| Radixin fluorescence intensity | 128 ± 12 | 126 ± 12 | ||||
| Moesin fluorescence intensity | 108 ± 10 | 108 ± 10 | ||||
| Growth cone area | 240 ± 20 | 265 ± 15 | ||||
| Mean number actin ribs | 38 ± 6 | 44 ± 8 | ||||
| Radixin–moesin antisense-treated | ||||||
| Radixin fluorescence intensity | 6 ± 0.5 | 44 ± 12 | 85 ± 10 | |||
| Moesin fluorescence intensity | 8 ± 0.9 | 38 ± 10 | 78 ± 12 | |||
| Growth cone area | 45 ± 5 | 86 ± 10 | 187 ± 14 | |||
| Mean number actin ribs | 8 ± 2 | 14 ± 4 | 26 ± 6 | |||
Cultures were treated with the radixin–moesin sense or antisense oligonucleotide mixture (2 μM) for 24 h; after that time the medium was replaced with a fresh one lacking oligonucleotides, and the cells were analyzed at different time intervals after the release of the treatment. Data are expressed as the mean ± SEM. Radixin and moesin levels were determined by quantitative fluorescence as described in the Materials and Methods section. Measurements were performed within the cell body, and are expressed in pixels. Area values are expressed in μm2. 100 cells were analyzed for each time point and experimental condition.