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Effects of inhibitory anti-hVPS34 antibodies on PDGF receptor trafficking. HepG2 cells expressing the human PDGF-β receptor were incubated with anti-receptor antibodies and PDGF at 4°C as above, then warmed to 37°C for 5 min. The cells were fixed and stained as in Fig. 5. The data is representative of three separate experiments. Images were collected using a Nikon Eclipse E-400 microscope with Nikon 60× NA 1.4 Planapo optics.
