Abstract
Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.
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Selected References
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- COONS A. H., SNYDER J. C. Localization of antigen in tissue cells; antigens of rickettsiae and mumps virus. J Exp Med. 1950 Jan 1;91(1):31-8, pl. doi: 10.1084/jem.91.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eagle H., Smith D. E., Vickers P. THE EFFECT OF COMBINATION WITH DIAZO COMPOUNDS ON THE IMMUNOLOGICAL REACTIVITY OF ANTIBODIES. J Exp Med. 1936 Apr 30;63(5):617–643. doi: 10.1084/jem.63.5.617. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hopkins S. J., Wormall A. Phenyl isocyanate protein compounds and their immunological properties. Biochem J. 1933;27(3):740–753. doi: 10.1042/bj0270740. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAPLAN M. E., COONS A. H., DEANE H. W. Localization of antigen in tissue cells; cellular distribution of pneumococcal polysaccharides types II and III in the mouse. J Exp Med. 1950 Jan 1;91(1):15-30, 4 pl. doi: 10.1084/jem.91.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pressman D., Hill R. F., Foote F. W. The Zone of Localization of Anti-Mouse-Kidney Serum as Determined by Radioautographs. Science. 1949 Jan 21;109(2821):65–66. doi: 10.1126/science.109.2821.65. [DOI] [PubMed] [Google Scholar]
- Smetana H. The Permeability of the Renal Glomeruli of Several Mammalian Species to Labelled Proteins. Am J Pathol. 1947 Mar;23(2):255–267. [PMC free article] [PubMed] [Google Scholar]