Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1959 Oct 31;110(5):827–844. doi: 10.1084/jem.110.5.827

SEQUENTIAL CELLULAR CHANGES PRODUCED BY TYPES 5 AND 7 ADENOVIRUSES IN HELA CELLS AND IN HUMAN AMNIOTIC CELLS

CYTOLOGICAL STUDIES AIDED BY FLUORESCEIN-LABELLED ANTIBODY

Georgiana S Boyer 1, Floyd W Denny Jr 1, Harold S Ginsberg 1
PMCID: PMC2137019  PMID: 13803575

Abstract

The sequential cytological changes which develop in tissue culture cells infected with adenovirus types 5 and 7 are described and compared with those produced by adenovirus types 1, 2, 3, and 4. The evidence that is presented indicates that types 1, 2, and 5 belong to one major subdivision of the adenovirus group and types 3, 4, and 7 to another. That the host cell nucleus is the principal site of adenovirus synthesis has been confirmed by fluorescent antibody studies. They have demonstrated the occurrence of type-specific adenovirus antigen in the characteristic intranuclear inclusions and other virus-induced structures reported to contain virus-like particles or shown by electronmicroscopy.

Full Text

The Full Text of this article is available as a PDF (1.8 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARSKI G. Caractère spécifique de la lésion cellulaire causée in vitro par les virus du groupe A.P.C. et sa valeur diagnostique. Ann Inst Pasteur (Paris) 1956 Nov;91(5):614–622. [PubMed] [Google Scholar]
  2. BLOCH D. P., MORGAN C., GODMAN G. C., HOWE C., ROSE H. M. A correlated histochemical and electron microscopic study of the intranuclear crystalline aggregates of adenovirus (RI-APC virus) in HeLa cells. J Biophys Biochem Cytol. 1957 Jan 25;3(1):1–8. doi: 10.1083/jcb.3.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOYER G. S., DENNY F. W., Jr, GINSBERG H. S. Intracellular localization of type 4 adenovirus. II. Cytological and fluorescein-labelled antibody studies. J Exp Med. 1959 Jan 1;109(1):85–96. doi: 10.1084/jem.109.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BOYER G. S., LEUCHTENBERGER C., GINSBERG H. S. Cytological and cytochemical studies of HeLa cells infected with adeno-viruses. J Exp Med. 1957 Mar 1;105(3):195–216. doi: 10.1084/jem.105.3.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DINGLE J. H., BADGER G. F., FELLER A. E., HODGES R. G., JORDAN W. S., Jr, RAMMELKAMP C. H., Jr A study of illness in a group of Cleveland families. I. Plan of study and certain general observations. Am J Hyg. 1953 Jul;58(1):16–30. doi: 10.1093/oxfordjournals.aje.a119587. [DOI] [PubMed] [Google Scholar]
  7. DUNNEBACKE T. H., ZITCER E. M. Preparation and cultivation of primary human amnion cells. Cancer Res. 1957 Dec;17(11):1043–1046. [PubMed] [Google Scholar]
  8. EAGLE H. The specific amino acid requirements of a human carcinoma cell (Stain HeLa) in tissue culture. J Exp Med. 1955 Jul 1;102(1):37–48. doi: 10.1084/jem.102.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GINSBERG H. S., BADGER G. F., DINGLE J. H., JORDAN W. S., Jr, KATZ S. Etiologic relationship of the RI-67 agent to acute respiratory disease (ARD). J Clin Invest. 1955 Jun;34(6):820–831. doi: 10.1172/JCI103137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GINSBERG H. S. Characteristics of the new respiratory viruses (adenoviruses). I. Qualitative and quantitative aspects of the neutralization reaction. J Immunol. 1956 Oct;77(4):271–278. [PubMed] [Google Scholar]
  11. GINSBERG H. S. Characteristics on the adenoviruses. III. Reproductive cycle of types 1 to 4. J Exp Med. 1958 Jan 1;107(1):133–152. doi: 10.1084/jem.107.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. GINSBERG H. S., GOLD E., JORDAN W. S., Jr Tryptose phosphate broth as supplementary factor for maintenance of HeLa cell tissue cultures. Proc Soc Exp Biol Med. 1955 May;89(1):66–71. doi: 10.3181/00379727-89-21718. [DOI] [PubMed] [Google Scholar]
  13. HAMLIN A., HARFORD C. G., PARKER E., VAN RAVENSWAAY T. Electron microscopy of HeLa cells infected with adenoviruses. J Exp Med. 1956 Sep 1;104(3):443–454. doi: 10.1084/jem.104.3.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KJELLEN L., LAGERMALM G., SVEDMYR A., THORSSON K. G. Crystalline-like patterns in the nuclei of cells infected with an animal virus. Nature. 1955 Mar 19;175(4455):505–506. doi: 10.1038/175505a0. [DOI] [PubMed] [Google Scholar]
  15. LAGERMALM G., KJELLEN L., THORSSON K. G., SVEDMYR A. Electron microscopy of HeLa cells infected with agents of the adenovirus (APC-RI-ARD) group. Arch Gesamte Virusforsch. 1957;7(3):221–241. doi: 10.1007/BF01240877. [DOI] [PubMed] [Google Scholar]
  16. LEUCHTENBERGER C., BOYER G. S. The occurrence of intranuclear crystals in living HeLa cells infected with adenoviruses. J Biophys Biochem Cytol. 1957 Mar 25;3(2):323–324. doi: 10.1083/jcb.3.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MORGAN C., GODMAN G. C., ROSE H. M., HOWE C., HUANG J. S. Electron microscopic and histochemical studies of an unusual crystalline protein occurring in cells infected by type 5 adenovirus. J Biophys Biochem Cytol. 1957 May 25;3(3):505–508. doi: 10.1083/jcb.3.3.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ORFILA J. Etude de l'action cytopathogène de l'adénovirus type 7, en cultures cellulaires. Ann Inst Pasteur (Paris) 1958 Jun;94(6):794–797. [PubMed] [Google Scholar]
  19. PEREIRA H. G., ALLISON A. C., BALFOUR B. Multiplication of adenovirus type 5 studied by infectivity titrations and by the fluorescent antibody technique. Virology. 1959 Mar;7(3):300–314. doi: 10.1016/0042-6822(59)90200-4. [DOI] [PubMed] [Google Scholar]
  20. PEREIRA H. G., KELLY B. Dose-response curves of toxic and infective actions of adenovirus in HeLa cell cultures. J Gen Microbiol. 1957 Oct;17(2):517–524. doi: 10.1099/00221287-17-2-517. [DOI] [PubMed] [Google Scholar]
  21. SCHERER W. F., SYVERTON J. T., GEY G. O. Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J Exp Med. 1953 May;97(5):695–710. doi: 10.1084/jem.97.5.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WELLER T. H., COONS A. H. Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro. Proc Soc Exp Biol Med. 1954 Aug-Sep;86(4):789–794. doi: 10.3181/00379727-86-21235. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES