Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1964 Oct 31;120(5):925–942. doi: 10.1084/jem.120.5.925

THE ROLE OF COMPLEMENT IN THE PASSIVE CUTANEOUS REACTION OF MICE

S Ben-Efraim 1, B Cinader 1
PMCID: PMC2137863  PMID: 14247729

Abstract

Intradermal injection of mice with ribonuclease antibody, followed by intravenous injection with ribonuclease, resulted in permeability increase, demonstrable by "blueing." The size of the blued area depends on the quantity of antibody injected and on the interval between the two injections. If antigen was injected first and antibody was injected subsequently, a similar increase in permeability was observed in animals having a complete complement system (MuB1-positive) and in animals which have a deficient complement system (MuB1-negative). Marked differences in response were observed between these two types of mice if antigen was injected some hours after the antibody. In MuB1-negative mice, a blueing reaction was not observed at intervals between injections (2½ hours if 3 µg N antibody and 15 hours if 25 µg N antibody were injected intradermally) at which MuB1-positive animals showed a marked permeability increase. At these intervals, blueing did occur in MuB1-negative animals if they were injected with the serum of MuB1-positive mice or with fresh guinea pig serum. Blueing was not induced if the serum of MuB1-negative mice or heated guinea pig serum was injected. The occurrence of two distinct phases of the cutaneous reaction, of which only one involves the complete hemolytic complement system, was deduced from these observations.

Full Text

The Full Text of this article is available as a PDF (951.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENEDICT A. A., TIPS R. L. Actively and passively induced Arthus reactions in the mouse. Proc Soc Exp Biol Med. 1954 Dec;87(3):618–622. doi: 10.3181/00379727-87-21463. [DOI] [PubMed] [Google Scholar]
  2. BIER O. G., SIQUEIRA M., OSLER A. G. Studies on the mechanism of hypersensitivity phenomena. I. The effect of in vivo antigen-antibody reactions on passive cutaneous anaphylaxis in the rat. Int Arch Allergy Appl Immunol. 1955;7(1):1–9. doi: 10.1159/000228200. [DOI] [PubMed] [Google Scholar]
  3. BIER O. G., SIQUEIRA M. Passive reversed cutaneous anaphylaxis to protein antigens; preliminary report. Int Arch Allergy Appl Immunol. 1955;6(4-6):391–395. doi: 10.1159/000228196. [DOI] [PubMed] [Google Scholar]
  4. BIER O. G., SIQUEIRA M. Prevention by intravenous injection of antigen and antibody of passive Arthus reaction to unrelated immune system. Proc Soc Exp Biol Med. 1959 Jul;101(3):502–505. doi: 10.3181/00379727-101-24996. [DOI] [PubMed] [Google Scholar]
  5. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis. J Exp Med. 1963 Jun 1;117:965–981. doi: 10.1084/jem.117.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CARTER B. G., CINADER B., ROSS C. A. Immunochemical analysis of the multiple forms of bovine ribonuclease. Ann N Y Acad Sci. 1961 Nov 2;94:1004–1030. doi: 10.1111/j.1749-6632.1961.tb35592.x. [DOI] [PubMed] [Google Scholar]
  7. CINADER B., DUBISKI S. AN APLHA-GLOBULIN ALLOTYPE IN THE MOUSE (MUB1). Nature. 1963 Nov 23;200:781–781. doi: 10.1038/200781a0. [DOI] [PubMed] [Google Scholar]
  8. CINADER B., DUBISKI S. EFFECT OF AUTOLOGOUS PROTEIN ON THE SPECIFICITY OF THE ANTIBODY RESPONSE: MOUSE AND RABBIT ANTIBODY TO MUBL. Nature. 1964 Apr 4;202:102–103. doi: 10.1038/202102a0. [DOI] [PubMed] [Google Scholar]
  9. CINADER B., DUBISKI S., WARDLAW A. C. DISTRIBUTION, INHERITANCE, AND PROPERTIES OF AN ANTIGEN, MUB1, AND ITS RELATION TO HEMOLYTIC COMPLEMENT. J Exp Med. 1964 Nov 1;120:897–924. doi: 10.1084/jem.120.5.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. CINADER B., PEARCE J. H. Immunochemical studies on bovine ribonuclease. Br J Exp Pathol. 1956 Dec;37(6):541–552. [PMC free article] [PubMed] [Google Scholar]
  11. Chase M. W. STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS : X. ANTIBODIES INDUCING IMMEDIATE-TYPE SKIN REACTIONS. J Exp Med. 1947 Nov 30;86(6):489–514. doi: 10.1084/jem.86.6.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. DAVIES G. E. INHIBITION OF GUINEA-PIG COMPLEMENT IN VITRO AND IN VIVO BY CARRAGEENIN. Immunology. 1963 Nov;6:561–568. [PMC free article] [PubMed] [Google Scholar]
  13. FRICK O. L., STIFFEL C., BIOZZI G. Studies on the effect of complement on anaphylaxis in the mouse. J Immunol. 1962 May;88:595–599. [PubMed] [Google Scholar]
  14. HERZENBERG L. A., TACHIBANA D. K., HERZENBERG L. A., ROSENBERG L. T. A gene locus concerned with hemolytic complement in Mus musculus. Genetics. 1963 May;48:711–715. doi: 10.1093/genetics/48.5.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HUMPHREY J. H. The mechanism of Arthus reactions. I. The role of polymorphonuclear leucocytes and other factors in reversed passive Arthus reactions in rabbits. Br J Exp Pathol. 1955 Jun;36(3):268–282. [PMC free article] [PubMed] [Google Scholar]
  16. ISHIZAKA T., ISHIZAKA K. Biological activities of aggregated gamma globulin. I. Skin reactive and complement-fixing properties of heat denatured gamma globulin. Proc Soc Exp Biol Med. 1959 Aug-Sep;101:845–850. doi: 10.3181/00379727-101-25116. [DOI] [PubMed] [Google Scholar]
  17. OSLER A. G., HAWRISIAK M. M., OVARY Z., SIQUEIRA M., BIER O. G. Studies on the mechanism of hypersensitivity phenomena. II. The participation of complement in passive cutaneous anaphylaxis of the albino rat. J Exp Med. 1957 Dec 1;106(6):811–834. doi: 10.1084/jem.106.6.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. OVARY Z., BENACERRAF B., BLOCH K. J. Properties of guinea pig 7S antibodies. II. Identification of antibodies involved in passive cutaneous and systemic anaphylaxis. J Exp Med. 1963 Jun 1;117:951–964. doi: 10.1084/jem.117.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. OVARY Z., BIER O. G. Quantitative studies on passive cutaneous anaphylaxis in the guinea pig and its relationship to the Arthus phenomenon. J Immunol. 1953 Jul;71(1):6–11. [PubMed] [Google Scholar]
  20. OVARY Z., BIER O. G. Quantitative study of Arthus reaction and of cutaneous anaphylaxis induced passively in the rat. Proc Soc Exp Biol Med. 1952 Dec;81(3):584–586. doi: 10.3181/00379727-81-19949. [DOI] [PubMed] [Google Scholar]
  21. OVARY Z., BRIOT M. Nouvelle méthode de dosage de l'anticorps anaphylactique et son rapport avec l'azote de l'anticorps. Ann Inst Pasteur (Paris) 1951 Dec;81(6):670–672. [PubMed] [Google Scholar]
  22. OVARY Z. Passive cutaneous anaphylaxis in the mouse. J Immunol. 1958 Oct;81(4):355–357. [PubMed] [Google Scholar]
  23. ROSENBERG L. T., TACHIBANA D. K. Activity of mouse complement. J Immunol. 1962 Dec;89:861–867. [PubMed] [Google Scholar]
  24. SUGAHARA T., ISHIZAKA K., ISHIZAKA T. BIOLOGIC ACTIVITIES OF AGGREGATED GAMMA-GLOBULIN. VI. AGGREGATED PRODUCTS OF RABBIT SERUM PROTEINS. J Immunol. 1963 Jun;90:960–970. [PubMed] [Google Scholar]
  25. TERRY W. D., BORSOS T., RAPP H. J. DIFFERENCES IN SERUM COMPLEMENT ACTIVITY AMONG INBRED STRAINS OF MICE. J Immunol. 1964 Apr;92:576–578. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES