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. 1968 Nov 30;128(6):1309–1325. doi: 10.1084/jem.128.6.1309

THE INTERACTION OF HUMAN MONOCYTES AND LYMPHOCYTES

Martin J Cline 1, Virginia C Swett 1
PMCID: PMC2138575  PMID: 5688078

Abstract

Monocytes isolated from the peripheral blood of tuberculin-positive and tuberculin-negative donors were exposed to PPD, extensively washed, and incubated with autologous or homologous lymphocytes. Lymphocyte transformation was measured morphologically and by incorporation of 14C-labeled thymidine. Monocytes from tuberculin-positive subjects induced transformation of autologous lymphocytes in 19 of 29 experiments. Studies to define the optimal conditions of exposure to monocytes to PPD and to autologous lymphocytes showed that viable, metabolically intact monocytes are required. A ratio of only 1 monocyte to 100 lymphocytes sufficed to induce transformation; neutrophils were inactive. In general, PPD-sensitized monocytes failed to induce transformation of homologous lymphocytes from either tuberculin-positive or tuberculin-negative subjects. Direct contact between monocytes and lymphocytes was required for consistent transformation, and islands of transforming lymphocytes were observed around a central core of monocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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