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. 1974 Feb 1;139(2):398–406. doi: 10.1084/jem.139.2.398

FUNCTIONAL CHARACTERISTICS OF PEYER'S PATCH LYMPHOID CELLS

I. INDUCTION OF HUMORAL ANTIBODY AND CELL-MEDIATED ALLOGRAFT REACTIONS

Martin F Kagnoff 1, Stephen Campbell 1
PMCID: PMC2139531  PMID: 4589991

Abstract

Peyer's patches from normal mice contain antigen-sensitive B and T cells, but lack the accessory adherent cell type(s) required both for the induction of humoral immune responses and for the induction of allograft reactions against cell surface alloantigens. Immune responsiveness can be restored to cultures of Peyer's patch cells by the addition of either APEC or ME. Peyer's patch B cells can be specifically induced by antigen to synthesize humoral antibody. Peyer's patch T cells can cooperate in B-cell induction and can be induced to mediate an allograft reaction against an allogeneic stimulus. Peyer's patch lymphoid aggregates appear to be a storehouse of antigen-sensitive cells sequestered in such a way as to lack an accessory cell type or factor required for induction,

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bienenstock J., Dolezel J. Peyer's patches: lack of specific antibody-containing cells after oral and parenteral immunization. J Immunol. 1971 Apr;106(4):938–945. [PubMed] [Google Scholar]
  2. Broome J. D., Jeng M. W. Promotion of replication in lymphoid cells by specific thiols and disulfides in vitro. Effects on mouse lymphoma cells in comparison with splenic lymphocytes. J Exp Med. 1973 Sep 1;138(3):574–592. doi: 10.1084/jem.138.3.574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brunner K. T., Mauel J., Rudolf H., Chapuis B. Studies of allograft immunity in mice. I. Induction, development and in vitro assay of cellular immunity. Immunology. 1970 Apr;18(4):501–515. [PMC free article] [PubMed] [Google Scholar]
  4. Chan E. L., Mishell R. I., Mitchell G. F. Cell interaction in an immune response in vitro: requirement for theta-carrying cells. Science. 1970 Dec 11;170(3963):1215–1217. doi: 10.1126/science.170.3963.1215. [DOI] [PubMed] [Google Scholar]
  5. Chanana A. D., Schaedeli J., Hess M. W., Cottier H. Predominance of theta-positive lymphocytes in gut-associated and peripheral lymphoid tissues of newborn mice. J Immunol. 1973 Jan;110(1):283–285. [PubMed] [Google Scholar]
  6. Chen C., Hirsch J. G. Restoration of antibody-forming capacity in cultures of nonadherent spleen cells by mercaptoethanol. Science. 1972 Apr 7;176(4030):60–61. doi: 10.1126/science.176.4030.60. [DOI] [PubMed] [Google Scholar]
  7. Cosenza H., Leserman L. D., Rowley D. A. The third cell type required for the immune response of spleen cells in vitro. J Immunol. 1971 Aug;107(2):414–421. [PubMed] [Google Scholar]
  8. Craig S. W., Cebra J. J. Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit. J Exp Med. 1971 Jul 1;134(1):188–200. doi: 10.1084/jem.134.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Feldmann M. Cell interactions in the immune response in vitro. II. The requirement for macrophages in lymphoid cell collaboration. J Exp Med. 1972 May 1;135(5):1049–1058. doi: 10.1084/jem.135.5.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hamaoka T., Katz D. H., Benacerraf B. Radioresistance of carrier-specific helper thymus-derived lymphocytes in mice. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3453–3458. doi: 10.1073/pnas.69.11.3453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heim L. R., McGarry M. P., Montgomery J. R., Trentin J. J., South M. A. Potentials of spleen, lymph node, and Peyer's patches to reconstitute lymphoid tissue and produce graft-versus-host reaction. Transplantation. 1972 Oct;14(4):418–423. doi: 10.1097/00007890-197210000-00002. [DOI] [PubMed] [Google Scholar]
  12. Henry C., Faulk W. P., Kuhn L., Yoffey J. M., Fudenberg H. H. Peyer's patches: immunologic studies. J Exp Med. 1970 Jun 1;131(6):1200–1210. doi: 10.1084/jem.131.6.1200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Joel D. D., Hess M. W., Cottier H. Magnitude and pattern of thymic lymphocyte migration in neonatal mice. J Exp Med. 1972 Apr 1;135(4):907–923. doi: 10.1084/jem.135.4.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Katz D. H., Paul W. E., Goidl E. A., Benacerraf B. Carrier function in anti-hapten antibody responses. 3. Stimulation of antibody synthesis and facilitation of hapten-specific secondary antibody responses by graft-versus-host reactions. J Exp Med. 1971 Feb 1;133(2):169–186. doi: 10.1084/jem.133.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kettman J., Dutton R. W. Radioresistance of the enhancing effect of cells from carrier-immunized mice in an in vitro primary immune response. Proc Natl Acad Sci U S A. 1971 Apr;68(4):699–703. doi: 10.1073/pnas.68.4.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lefkovits I. In vitro complementation experiments with nude mice. I. The allogeneic effect in the antibody response to sheep red cells. Eur J Immunol. 1973 Jul;3(7):397–401. doi: 10.1002/eji.1830030705. [DOI] [PubMed] [Google Scholar]
  17. Levin D. M., Rosenstreich D. L., Reynolds H. Y. Immunologic responses in the gastrointestinal tract of the guinea pig. I. Characterization of Peyer's patch cells. J Immunol. 1973 Sep;111(3):980–983. [PubMed] [Google Scholar]
  18. MacDonald H. R., Phillips R. A., Miller R. G. Allograft immunity in the mouse. II. Physical studies of the development of cytotoxic effector cells from their immediate progenitors. J Immunol. 1973 Aug;111(2):575–589. [PubMed] [Google Scholar]
  19. Miller J. F., Mitchell G. F. Thymus and antigen-reactive cells. Transplant Rev. 1969;1:3–42. doi: 10.1111/j.1600-065x.1969.tb00135.x. [DOI] [PubMed] [Google Scholar]
  20. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mosier D. E., Coppleson L. W. A THREE-CELL INTERACTION REQUIRED FOR THE INDUCTION OF THE PRIMARY IMMUNE RESPONSE in vitro. Proc Natl Acad Sci U S A. 1968 Oct;61(2):542–547. doi: 10.1073/pnas.61.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Perey D. Y., Guttmann R. D. Peyer's patch cells. Absence of graft-versus-host reactivity in mice and rats. Lab Invest. 1972 Oct;27(4):427–433. [PubMed] [Google Scholar]
  23. Raff M. C., Owen J. J. Thymus-derived lymphocytes: their distribution and role in the development of peripheral lymphoid tissues of the mouse. Eur J Immunol. 1971 Jan;1(1):27–30. doi: 10.1002/eji.1830010105. [DOI] [PubMed] [Google Scholar]
  24. Wagner H., Feldmann M., Boyle W., Schrader J. W. Cell-mediated immune response in vitro. 3. The requirement for macrophages in cytotoxic reactions against cell-bound and subcellular alloantigens. J Exp Med. 1972 Aug 1;136(2):331–343. doi: 10.1084/jem.136.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Waksman B. H. The homing pattern of thymus-derived lymphocytes in calf and neonatal mouse Peyer's patches. J Immunol. 1973 Sep;111(3):878–884. [PubMed] [Google Scholar]
  26. Watson J., Epstein R. The role of humoral factors in the initiation of in vitro primary immune responses. I. Effects of deficient fetal bovine serum. J Immunol. 1973 Jan;110(1):31–42. [PubMed] [Google Scholar]

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