Table IV.
Comparison of the Nucleation and “Consumption” of MTs at the Centrosome to the Appearance and Disappearance of Plus Ends in the Lamella
| MTs/centrosome (MTs/5 min) | MT nucleations (MTs/min) | MTs depolymerizing to centrosome (MTs/min) | MT releases from centrosome (MTs/min) | |||||
|---|---|---|---|---|---|---|---|---|
| Centrosome (n = 7 cells) | 22.4 ± 9.6 | 2.9 ± 2.4 | 2.8 ± 2.6 | 0.02 ± 0.04 | ||||
| MTs/lamella (MTs/5 min) | MT + ends entering lamella (ends/min) | MT + ends depolymerizing out of lamella (ends/min) | MT − ends entering lamella* (ends/min) | |||||
| 90 × 30 μm Lamella‡ (n = 19 cells) | >90 | 6.9 ± 4.5 | 0.6 ± 0.9 | 0.9 ± 0.6 |
*
Minus ends could enter the field either by breaking within the field of view or depolymerizing towards the leading edge from outside the field of view.
‡
Values were determined for a 30 × 30 microscopic field of view of the lamella and multiplied by three to give a value for a lamella of typical length.