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. 1998 Aug 10;142(3):815–825. doi: 10.1083/jcb.142.3.815

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Effects of the expression of cRac1A and cRac1B GTPases on neuronal morphology. Retinal neurons cultured overnight on polylysine- and laminin-coated coverslips were transfected with pFLAG-LacZ (a and d), pFLAG-cRac1B (b, c, e, and f), or pFLAG-cRac1A (g–i). After 1 d more in culture, cells were processed for immunofluorescence using the anti–β-galactosidase mAb (a), the anti–Flag-M5 mAb (b, f, g, and i), the anti-cRac1B polyclonal antibody (c), and the polyclonal antibody against the 200-kD neurofilament protein (d, e, and h). The same cells are shown in a and d; in b and e; and in g and h. Bar, 10 μm.