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. 2000 Sep 18;150(6):1507–1513. doi: 10.1083/jcb.150.6.1507

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Apg1-associating proteins are required for Apg1 activation. A, Wild-type (TN125, lane 1), apg1Δ (YYK126, lane 2), apg13Δ (YYK130, lane 3), and apg17Δ (YYK121, lane 4) cells were analyzed with anti-API blot (top) and ALP assay (bottom). B, Wild-type (KA311A), apg13Δ (YYK119), apg17Δ (YYK111), and cvt9Δ (YYK107) cells expressing ^HAApg1 were treated with rapamycin (0.2 μg/ml, 1 h) and analyzed by kinase assay.

Apg1-associating proteins are required for Apg1 activation. A, Wild-type (TN125, lane 1), apg1Δ (YYK126, lane 2), apg13Δ (YYK130, lane 3), and apg17Δ (YYK121, lane 4) cells were analyzed with anti-API blot (top) and ALP assay (bottom). B, Wild-type (KA311A), apg13Δ (YYK119), apg17Δ (YYK111), and cvt9Δ (YYK107) cells expressing ^HAApg1 were treated with rapamycin (0.2 μg/ml, 1 h) and analyzed by kinase assay.