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. 2001 Sep 17;154(6):1209–1224. doi: 10.1083/jcb.200106089

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Copyright © 2001, The Rockefeller University Press

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Figure 4. — Time-lapse video microscopy of Hip1R–YFP and DsRed–clathrin-LCa in live cells. These proteins display almost identical behavior at the cell cortex. Images were taken at 3 s intervals over 60 frames, where the lag between Hip1R and clathrin visualization is 1.5 s. (a–c) These images show a low magnification overview of a Cos-7 cell coexpressing Hip1R–YFP (green) and DsRed–clathrin-LCa (red). (a) Hip1R–YFP. (b) DsRed–clathrin-LCa. (c) Merge. (d–f) Selected images at the indicated times show that Hip1R–YFP and DsRed–clathrin-LCa appear (arrow) and disappear (arrowhead) at the same sites with indistinguishable timing. (d) Hip1R–YFP. (e) DsRed-clathrin-LCa. (f) Merge. A video containing images from this figure is available at http://www.jcb.org/content/vol154/issue6. Bar, 5 μm.