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. 2001 Nov 26;155(5):733–738. doi: 10.1083/jcb.200105103

Table I. Semiquantitative analysis of Purkinje cell engraftment.

Mouse GFP labelling Time
post-BMT		 GFP+ microglia GFP+ cells
Perivascular/leptomeningeal		 GFP+Purkinje cells Total
Purkinje cells
mo
1 Retroviral 12 1,107 2,515 10 13,519
2 Retroviral 12 2,064 2,989 10 13,691
3 Transgenic 10 747 1,331 2 13,110

10–12 mo after transplantation of BM cells expressing GFP under the control of a retroviral or β-actin promoter, every seventh cerebellar section of the chimeric mice was analyzed for GFP-expressing cells. Purkinje cells and microglia were identified by their morphology and immunoreactivity for calbindin/GAD and Iba1, respectively. GFP-positive cells located in perivascular and leptomeningeal sites were also evaluated.