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. 2004 Oct 11;167(1):87–98. doi: 10.1083/jcb.200405100

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — Cells lacking Gem1p grow slowly on synthetic glycerol medium. Strains (JSY7000 GEM1 or JSY7002 gem1Δ) with the indicated plasmid were spotted on SDextrose or SGlycerol lacking the appropriate amino acid and grown for 3 d at the indicated temperature. (A) Gem1p expressed from its own promoter (pRS416-GEM1) complements the gem1Δ glycerol growth defect. (B) GFP-Gem1p expressed from the TPI promoter (pYX142-GFP-GEM1) partially complements the gem1Δ glycerol growth defect.