Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Sep 30;158(7):1263–1275. doi: 10.1083/jcb.200203043

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Photobleaching reveals that ZAP-70 equilibrates between signaling complexes. (A and B) ZAP-70–EGFP-expressing Jurkat T cells were plated on coverslips and allowed to develop contacts for either 2 or 20 min. Contacts were imaged for 20 s, photobleached within a specific region of interest (ROI, red boxes), and monitored for the recovery of fluorescence in the bleached ROI. Images were obtained every 2.5 s. (C) Mean fluorescence recovery traces from cells plated for 2 min before photobleaching (n = 6). (D) Mean fluorescence recovery traces from cells plated for 20 min before photobleaching (n = 6). (E) The extent of fluorescence recovery in bleached ROIs as a function of the age of the observed contacts at the time of photobleaching. In each case, half-maximal recovery is observed within 7.5 to 10 s.