Figure 3.
DNup214 is mislocalized in mbo mutants. (A) DNup214 localization in fat body cells of wild-type, mbo mutants, and mbo larvae overexpressing DNup88. The top row shows DNup214 staining (red), and the bottom row shows DAPI. Confocal sections are shown in the insets. (B) Confocal sections of fat body cells from wild-type and mbo larvae stained with anti-mAb414 and anti-Nup153. Bars (A and B), 2 μm. (C) Western blot of S2 cells before (−) and after (+) Nup214 RNAi probed with the anti-DNup214 antibody. β-Tubulin is a loading control.