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. 2000 Nov 27;151(5):1025–1034. doi: 10.1083/jcb.151.5.1025

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The Cvt bypass phenotype is correlated with partial survival under nitrogen starvation conditions. A, Immunoblots of prAPI maturation in nitrogen starvation medium. SEY6210 (wild-type), THY119 (cvt3), WPHYD7 (aut7Δ), WPHYD2 (aut2Δ), and HAY395 (apg1Δ) strains were grown to midlog phase and shifted to SD-N medium for 0, 1, 2, 4, and 6 h. At each time point, 3 A₆₀₀ units of cells were precipitated in 10% TCA, washed twice with acetone, and processed for Western blotting as described in Materials and Methods. B, Viability of yeast strains in nitrogen starvation medium. SEY6210 (wild-type), THY119 (cvt3), WPHYD7 (aut7Δ), WPHYD2 (aut2Δ), and HAY395 (apg1Δ) strains were grown to midlog phase in SMD and transferred to SD-N medium. Aliquots were removed at the indicated times and spread onto YPD plates in triplicate. The number of colonies was counted after two to three days.