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. 1985 Sep 1;162(3):902–916. doi: 10.1084/jem.162.3.902

A common major surface antigen on amastigotes and promastigotes of Leishmania species

PMCID: PMC2187815  PMID: 4031788

Abstract

Enzymatic surface iodination and biosynthetic labeling with [35S]methionine, combined with immunoprecipitation by sera from patients with different forms of Leishmaniasis revealed a 65,000 Mr glycoprotein as the immunodominant moiety in promastigotes and amastigotes of the nine Leishmania species and isolates examined. Sera from patients with one form of Leishmaniasis recognized this component strongly, not only in the homologous, but also in the heterologous species. In addition to the crossreactivity displayed by immune sera, the 65,000 Mr glycoprotein (gp) common to all Leishmania species presented a characteristic shift to Mr 50,000 when samples were run in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing conditions. These results are in agreement with our previous studies (7), where a simple and similar profile was obtained for several geographic isolates of L. donovani, with a major surface glycoprotein of 65,000 Mr displaying the same characteristics described here. The structural similarity of the major 65,000 Mr gp of the six Leishmania species was demonstrated by Cleveland mapping. It is suggested that immunological specificities may be contributed by minor differences in glycosylation of this molecule. In keeping with recent data (13-15), where strong cross protection among different Leishmania species has been obtained by prophylactic immunization with irradiated whole promastigotes, this glycoprotein may be a good candidate for an antigen to be used for immunoprophylaxis of all forms of Leishmaniasis.

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Selected References

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  1. ADLER S. LEISHMANIA. Adv Parasitol. 1964;2:35–96. doi: 10.1016/s0065-308x(08)60586-2. [DOI] [PubMed] [Google Scholar]
  2. Alexander J., Phillips R. S. Leishmania tropica and Leishmania mexicana: cross-immunity in mice. Exp Parasitol. 1978 Jun;45(1):93–100. doi: 10.1016/0014-4894(78)90049-8. [DOI] [PubMed] [Google Scholar]
  3. Barker D. C., Butcher J. The use of DNA probes in the identification of leishmanias: discrimination between isolates of the Leishmania mexicana and L. braziliensis complexes. Trans R Soc Trop Med Hyg. 1983;77(3):285–297. doi: 10.1016/0035-9203(83)90146-3. [DOI] [PubMed] [Google Scholar]
  4. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  5. Chance M. L., Peters W., Shchory L. Biochemical taxonomy of Leishmania. I. Observations on DNA. Ann Trop Med Parasitol. 1974 Sep;68(3):307–316. [PubMed] [Google Scholar]
  6. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  7. Coelho M. de V., Coutinho-Abath E. Experimental cutaneous leishmaniasis. I. Infection of albino mice and Syrian hamsters by Leishmania mexicana. Rev Inst Med Trop Sao Paulo. 1965 May-Jun;7(3):136–144. [PubMed] [Google Scholar]
  8. Fong D., Chang K. P. Surface antigenic change during differentiation of a parasitic protozoan, Leishmania mexicana: Identification by monoclonal antibodies. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7366–7370. doi: 10.1073/pnas.79.23.7366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gardiner P. R., Dwyer D. M. Radioiodination and identification of externally disposed membrane components of Leishmania tropica. Mol Biochem Parasitol. 1983 Aug;8(4):283–295. doi: 10.1016/0166-6851(83)90075-0. [DOI] [PubMed] [Google Scholar]
  10. Gardiner P. R., Jaffe C. L., Dwyer D. M. Identification of cross-reactive promastigote cell surface antigens of some leishmanial stocks by 125I labeling and immunoprecipitation. Infect Immun. 1984 Feb;43(2):637–643. doi: 10.1128/iai.43.2.637-643.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Handman E., Curtis J. M. Leishmania tropica: surface antigens of intracellular and flagellate forms. Exp Parasitol. 1982 Oct;54(2):243–249. doi: 10.1016/0014-4894(82)90133-3. [DOI] [PubMed] [Google Scholar]
  12. Handman E., Hocking R. E. Stage-specific, strain-specific, and cross-reactive antigens of Leishmania species identified by monoclonal antibodies. Infect Immun. 1982 Jul;37(1):28–33. doi: 10.1128/iai.37.1.28-33.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Handman E., Mitchell G. F., Goding J. W. Identification and characterization of protein antigens of Leishmania tropica isolates. J Immunol. 1981 Feb;126(2):508–512. [PubMed] [Google Scholar]
  14. Harrison E. H., Bowers W. E. Characterization of rat lymphocyte cell membranes by analytical isopycnic centrifugation. J Biol Chem. 1983 Jun 10;258(11):7134–7140. [PubMed] [Google Scholar]
  15. Hernández A. G. Leishmanial excreted factors and their possible biological role. Ciba Found Symp. 1983;99:138–156. [PubMed] [Google Scholar]
  16. Hodgkinson V. H., Herman R. In vivo assay of viability of amastigotes of Leishmania donovani. J Parasitol. 1980 Apr;66(2):245–249. [PubMed] [Google Scholar]
  17. Howard J. G., Liew F. Y., Hale C., Nicklin S. Prophylactic immunization against experimental leishmaniasis. II. Further characterization of the protective immunity against fatal Leishmania tropica infection induced by irradiated promastigotes. J Immunol. 1984 Jan;132(1):450–455. [PubMed] [Google Scholar]
  18. Howard J. G., Liew F. Y. Mechanisms of acquired immunity in leishmaniasis. Philos Trans R Soc Lond B Biol Sci. 1984 Nov 13;307(1131):87–98. doi: 10.1098/rstb.1984.0111. [DOI] [PubMed] [Google Scholar]
  19. Howard J. G., Nicklin S., Hale C., Liew F. Y. Prophylactic immunization against experimental leishmaniasis: I. Protection induced in mice genetically vulnerable to fatal Leishmania tropica infection. J Immunol. 1982 Nov;129(5):2206–2212. [PubMed] [Google Scholar]
  20. Hubbard A. L., Cohn Z. A. Externally disposed plasma membrane proteins. I. Enzymatic iodination of mouse L cells. J Cell Biol. 1975 Feb;64(2):438–460. doi: 10.1083/jcb.64.2.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Keithly J. S., Langreth S. G. Inefficacy of metronidazole in experimental infections of Leishmania donovani, L. mexicana, and Trypanosoma brucei brucei. Am J Trop Med Hyg. 1983 May;32(3):485–496. doi: 10.4269/ajtmh.1983.32.485. [DOI] [PubMed] [Google Scholar]
  22. Kreutzer R. D., Christensen H. A. Characterization of Leishmania spp. by isozyme electrophoresis. Am J Trop Med Hyg. 1980 Mar;29(2):199–208. doi: 10.4269/ajtmh.1980.29.199. [DOI] [PubMed] [Google Scholar]
  23. Lainson R., Shaw J. J. Leishmaniasis in Brazil: XII. Observations on cross-immunity in monkeys and man infected with Leishmania mexicana mexicana, L. m. amazonensis, L. braziliensis braziliensis, L. b. guyanensis and L. b. panamensis. J Trop Med Hyg. 1977 Feb;80(2):29–35. [PubMed] [Google Scholar]
  24. Lainson R., Shaw J. J. Studies on the immunology and serology of leishmaniasis. 3. on the cross-immunity between Panamanian cutaneous leishmaniasis and Leishmania mexicana infection in man. Trans R Soc Trop Med Hyg. 1966;60(4):533–535. doi: 10.1016/0035-9203(66)90279-3. [DOI] [PubMed] [Google Scholar]
  25. Lainson R., Ward R. D., Shaw J. J. Leishmania in phlebotomid sandflies: VI. Importance of hindgut development in distinguishing between parasites of the Leishmania mexicana and L. braziliensis complexes. Proc R Soc Lond B Biol Sci. 1977 Nov 14;199(1135):309–320. doi: 10.1098/rspb.1977.0141. [DOI] [PubMed] [Google Scholar]
  26. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  27. Marsden P. D., Cuba C. C., Barreto A. C., Sampaio R. N., Rocha R. A. Nifurtimox in the treatment of South American leishmaniasis. Trans R Soc Trop Med Hyg. 1979;73(4):391–394. doi: 10.1016/0035-9203(79)90161-5. [DOI] [PubMed] [Google Scholar]
  28. McMahon-Pratt D., Bennett E., David J. R. Monoclonal antibodies that distinguish subspecies of Leishmania braziliensis. J Immunol. 1982 Sep;129(3):926–927. [PubMed] [Google Scholar]
  29. Neville D. M., Jr, Glossmann H. Molecular weight determination of membrane protein and glycoprotein subunits by discontinuous gel electrophoresis in dodecyl sulfate. Methods Enzymol. 1974;32:92–102. doi: 10.1016/0076-6879(74)32012-5. [DOI] [PubMed] [Google Scholar]
  30. Nogueira N., Chaplan S., Tydings J. D., Unkeless J., Cohn Z. Trypanosoma cruzi. Surface antigens of blood and culture forms. J Exp Med. 1981 Mar 1;153(3):629–639. doi: 10.1084/jem.153.3.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Nogueira N., Unkeless J., Cohn Z. Specific glycoprotein antigens on the surface of insect and mammalian stages of Trypanosoma cruzi. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1259–1263. doi: 10.1073/pnas.79.4.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Pérez H., Arredondo B., Machado R. Leishmania mexicana and Leishmania tropica: cross immunity in C57BL/6 mice. Exp Parasitol. 1979 Aug;48(1):9–14. doi: 10.1016/0014-4894(79)90049-3. [DOI] [PubMed] [Google Scholar]
  33. Schneider C. R., Hertig M. Immunodiffusion reactions of Panamanian Leishmania. Exp Parasitol. 1966 Feb;18(1):25–34. doi: 10.1016/0014-4894(66)90004-x. [DOI] [PubMed] [Google Scholar]
  34. Stauber L. A. Characterization of strains of Leishmania donovani. Exp Parasitol. 1966 Feb;18(1):1–11. doi: 10.1016/0014-4894(66)90002-6. [DOI] [PubMed] [Google Scholar]
  35. Unkeless J. C. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979 Sep 19;150(3):580–596. doi: 10.1084/jem.150.3.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. de Ibarra A. A., Howard J. G., Snary D. Monoclonal antibodies to Leishmania tropica major: specificities and antigen location. Parasitology. 1982 Dec;85(Pt 3):523–531. doi: 10.1017/s0031182000056304. [DOI] [PubMed] [Google Scholar]

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