Table 2.
Saccharomyces cerevisiae Strains Used in This Study
| Strain | Description | Reference |
|---|---|---|
| SNY36-9A | MAT a his3-Δ200 leu2-3,112 pho8Δ::ADE2 suc2-Δ9 trp1-901 ura3-52 | Nothwehr et al. 1995 |
| SNY80 | SNY36-9A vps35Δ::HIS3 | This study |
| SNY110 | SNY36-9A vps35-101 | This study |
| SNY114 | SNY36-9A vps35-105 | This study |
| AHY81 | SNY36-9A (converted to diploid) vps35-105/vps35-105 vps10/vps10 | This study |
| SNY71 | SNY36-9A pep4Δ::LEU2 | This study |
| SHY28 | SNY36-9A vps10-Q 1499 L | This study |
| SHY29 | SNY36-9A vps10-Q 1499 Lvps35Δ::HIS3 | This study |
| SHY30 | SNY36-9A vps10-Q 1499 Lvps35-105 | This study |
| SNY132 | SNY36-9A pep4Δ::LEU2 VPS10::HA vps35Δ::HIS3 | This study |
| SNY133 | SNY36-9A pep4Δ::TRP1 VPS10::HA vps35-105 | This study |
| SNY135 | SNY36-9A pep4Δ::LEU2 vps10-Q 1499 L::HA | This study |
| SNY136 | SNY36-9A pep4Δ::LEU2 vps10-Q 1499 L::HA vps35Δ::HIS3 | This study |
| SNY137 | SNY36-9A pep4Δ::TRP1 vps10-Q 1499 L::HA vps35-105 | This study |
| W303-1A | MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | R. Rothstein |
| AHY63 | W303-1A pep4-ΔH3 pho8Δ::ADE2 | This study |
| AHY66 | W303-1A pep4-ΔH3 pho8Δ::ADE2 vps35Δ::HIS3 | This study |
| SNY102 | W303-1A pep4-ΔH3 pho8Δ::ADE2 vps35-101 | This study |
| SNY126 | W303-1A pep4-ΔH3 pho8Δ::ADE2 vps29::HIS3 | This study |
| SNY128 | W303-1A pep4-ΔH3 pho8Δ::ADE2 VPS10::HA vps27Δ::LEU2 | This study |
| SNY129 | W303-1A pep4-ΔH3 pho8Δ::ADE2 VPS10::HA vps27Δ::LEU2 vps35Δ::HIS3 | This study |
| SNY134 | W303-1A pep4-ΔH3 pho8Δ::ADE2 vps10-Q 1499 L::HA vps27Δ::LEU2 vps35Δ::HIS3 | This study |
| SEY6210 | MATα his3-Δ200 leu2-3,112 lys2-801 suc2-Δ9 trp1-Δ901 ura3-52 | Robinson et al. 1988 |
| SNY132 | SEY6210 pep4Δ::TRP1 pho8Δ::LEU2 VPS10::HA vps35Δ::HIS3 | This study |
| SNY136 | SEY6210 pep4Δ::TRP1 pho8Δ::LEU2 vps10-Q 1499 L::HA vps35Δ::HIS3 | This study |
| PBY1 | SEY6210 pep4Δ::TRP1 pho8Δ::LEU2 vps35Δ::HIS3 | This study |
| PBY2 | SEY6210 pep4Δ::TRP1 pho8Δ::LEU2 vps35-101 | This study |
| SHY26 | SEY6210 pho8-ΔX vps27Δ::LEU2 | This study |
| SNY105 | SEY6210 (converted to diploid) pho8Δ::LEU2/pho8Δ::LEU2 vps35-101/vps35-101 | This study |