Table III.
Strain and Tissue Distribution, and MHC Selection of mAV19-AJ33+ T Cells
| Strain | Haplotype | Lymph nodes | Spleen | PBLs | Thymus | Bone marrow | Liver | IELs | Lamina propria | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BALB/c | H-2d | +++ | + | +++ | + | + | − | − | − | |||||||||
| I-A+/I-E+ | ||||||||||||||||||
| DBA/2 | H-2d | +++ | ||||||||||||||||
| I-A+/I-E+ | ||||||||||||||||||
| CBA | H-2k | +++ | + | |||||||||||||||
| I-A+/I-E+ | ||||||||||||||||||
| 129 | H-2b | + | +++ | |||||||||||||||
| I-A+/I-E− | ||||||||||||||||||
| C57BL/6 | H-2b | +++ | +++ | + | ||||||||||||||
| I-A+/I-E− | ||||||||||||||||||
| NUDE B6 | H-2b | − | − | |||||||||||||||
| I-A+/I-E− | ||||||||||||||||||
| B6 | I-Ab−/− | β2m−/− | Kb−/−/Db−/− | TAP−/− | CD1−/− | CD8−/− | ||||||||||||
| mAV19-AJ33 | ++ | ++ | − | ++ | +++ | ++ | ++ | |||||||||||
| (fold increase | (2) | (4) | (8) | (2) | (4) | |||||||||||||
| compared with B6) | ||||||||||||||||||
| mAV14-AJ18 | ++ | ++ | − | ++ | ++ | − | ++ |
DN α/β+/+ T cells were purified from the indicated organs in different mouse strains. Expression of the invariant mAV19-AJ33 TCR α chain was estimated by quantitative kinetic PCR and confirmed by polyclonal sequencing (see Materials and Methods, and Figs. 1 and 2). Tissue distribution results are compared with the expression in sorted CD4+ α/β+ T cells: +++, high expression (>3-cycle shift to the left); +, no increase; −, absence as judged by polyclonal sequencing of mAV19-AJ33 amplicons. Selection results (bottom) in the lymph nodes of the different MHC-deficient mice are compared with the expression in DN cells from B6 lymph nodes using quantitative PCR. All of the results were also confirmed by polyclonal sequencing after mAV19-Cα, or -AJ33 amplification as appropriate.