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. 2001 Oct 1;194(7):915–926. doi: 10.1084/jem.194.7.915

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© 2001 The Rockefeller University Press

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Rac protein expression and T cell development in Rac2^−/− and Rac2^+/+ mice. (A) Rac1 and Rac2 protein expression in subpopulation of thymocytes and mature peripheral T cells. 50 μg of cleared cell lysate from each cell population was used for Western blotting. The same blot probed for Rac1 was stripped and reprobed for Rac2. Lane 1 and 7, mature CD4⁺ cells; lane 2 and 8, mature CD8 cells; lane 3 and 9, CD4⁺CD8⁺ thymocytes; lane 4 and 10, CD4⁻CD8⁻ thymocytes; lane 5 and 11, CD4⁺ thymocytes; lane 6 and 12, CD8⁺ thymocytes. The rac2 antibody weakly cross reacts with another protein in lysates from Rac2^−/− cells and the control cells (reference 9). Data represents one of two experiments. (B) Lymph node cells or thymocytes were isolated from Rac2^−/− mice and its littermate Rac2^+/+ mice. The expression of CD4 and CD8 was examined by two-color flow cytometry.

Rac protein expression and T cell development in Rac2^−/− and Rac2^+/+ mice. (A) Rac1 and Rac2 protein expression in subpopulation of thymocytes and mature peripheral T cells. 50 μg of cleared cell lysate from each cell population was used for Western blotting. The same blot probed for Rac1 was stripped and reprobed for Rac2. Lane 1 and 7, mature CD4⁺ cells; lane 2 and 8, mature CD8 cells; lane 3 and 9, CD4⁺CD8⁺ thymocytes; lane 4 and 10, CD4⁻CD8⁻ thymocytes; lane 5 and 11, CD4⁺ thymocytes; lane 6 and 12, CD8⁺ thymocytes. The rac2 antibody weakly cross reacts with another protein in lysates from Rac2^−/− cells and the control cells (reference 9). Data represents one of two experiments. (B) Lymph node cells or thymocytes were isolated from Rac2^−/− mice and its littermate Rac2^+/+ mice. The expression of CD4 and CD8 was examined by two-color flow cytometry.