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. 2001 Oct 1;194(7):915–926. doi: 10.1084/jem.194.7.915

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Reduced calcium mobilization in Rac2^−/− T cells. (A) AND TCR transgenic CD4 cells were loaded with the Ca²+ sensitive fluorochrome, fluo-3, and stimulated with splenic APCs prepulsed with 50 μg/ml or 0.5 μg/ml MCC peptide. (B) CD4 T cells loaded with fluo-3 were stimulated with 0.1 or 1 μg /ml anti-CD3. The percentage of responding cells and the pattern of response in individual T cells was examined using a video laser cytometer. Each graph indicates the pattern of Ca²+ mobilization in a field of 40 to 50 cells, in which each line represents the average fluorescent intensity of an individual T cell over time. The first arrow indicates the addition of APC pulsed with peptide or cross-linking antibody, and the second arrow indicates the addition of ionomycin (666 ng/ml). S stands for sustained response, i.e., increased calcium concentration lasting >5 min. T stands for transient response, i.e., calcium increase lasting less than 5 min.

Reduced calcium mobilization in Rac2^−/− T cells. (A) AND TCR transgenic CD4 cells were loaded with the Ca²+ sensitive fluorochrome, fluo-3, and stimulated with splenic APCs prepulsed with 50 μg/ml or 0.5 μg/ml MCC peptide. (B) CD4 T cells loaded with fluo-3 were stimulated with 0.1 or 1 μg /ml anti-CD3. The percentage of responding cells and the pattern of response in individual T cells was examined using a video laser cytometer. Each graph indicates the pattern of Ca²+ mobilization in a field of 40 to 50 cells, in which each line represents the average fluorescent intensity of an individual T cell over time. The first arrow indicates the addition of APC pulsed with peptide or cross-linking antibody, and the second arrow indicates the addition of ionomycin (666 ng/ml). S stands for sustained response, i.e., increased calcium concentration lasting >5 min. T stands for transient response, i.e., calcium increase lasting less than 5 min.