Figure 1.
Pfnbp1 shares features with members of the Pvrbp gene family. (A) PCR using primers flanking the putative Pfnbp1 intron. Template was either P. falciparum FVO gDNA (lane 1), cDNA from P. falciparum FVO RNA (lane 2), or reverse transcription reaction performed in absence of reverse transcriptase (lane 3). (B) Sequence of the Pfnbp1 intervening sequence and consensus splice sites (arrowed). (C) Diagram showing gene structure and encoded protein features of Pfnbp1 shared by other PvRBP super family members. The position of the fragment used to raise antiserum (N1) is also noted. Arrows denote approximate positions of potential frameshift mutations in 3D7 and 7G8 strains. (D) Phylogenetic relationships of the known members of the RBL superfamily as determined by Neighbor-Joining following a Poisson model with pairwise deletion.