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. 1999 May 17;189(10):1631–1638. doi: 10.1084/jem.189.10.1631

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Analysis of β7 integrin expression in OT-I T cells lacking either β7 or αE chains. Lymphocytes from MLN of OT-I, OT-I-αE^−/−, or OT-I-β7^−/− mice were examined for expression of Vα2 and Vβ5 or for CD8 and β7 by fluorescence flow cytometry. Similar staining was observed with PLN cells (data not shown). Top panel, analysis of Vα2 and Vβ5 expression of gated CD8⁺ LN cells from OT-I mice. Similar results were obtained from analysis of CD8⁺ cells from all three mouse strains. MFC, mean fluorescence channel.