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. 2002 Sep 16;196(6):781–791. doi: 10.1084/jem.20020186

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — HisRS-induced chemotaxis is inhibited by pertussis toxin. Primary lymphocytes were incubated with increasing amounts of pertussis toxin for 1 h before being added to the upper wells of a chemotaxis chamber. Compared with binding media control, the HRS-induced migration was significantly reduced (unpaired Student t test, P < 0.0001) at 10 ng/ml. Higher concentration of pertussis toxin begins to be toxic and reduce spontaneous migration. Each condition was performed in triplicate. Each assay was performed a minimum of three times.