Table II.
Changes in Relative Serum Affinity after Adoptive Transfer of Memory B Lymphocytes
| Mean ΔKD (BIAcore)
|
||
|---|---|---|
| Chimera | 1–12 wk | 14–16 wk |
| WT | 1.6 × 10−8 * | 1.5 × 10−8 * |
| Cr2−/− | 0.8 × 10−8 | 1.4 × 10−8 |
| FcγRIIB−/− | 0.2 × 10−8 | 4.8 × 10−8 * |
Mean changes in relative affinity (ΔKD; left side of table) were determined by calculating equilibrium dissociation constants (KD) for each time point serum sample, followed by subtraction of starting KD values 2–3 wk after cell transfer (n = 20 for WT, 11 each for Cr2−/− and FcγRIIB−/− chimeras). Results are summarized from two independent experiments. *, statistically significant differences upon comparison to mean 2–3-wk time point values (P < 0.03 for WT chimeras and P < 0.003 for FcγRIIB−/− chimeras).