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. 1997 Jan 20;185(2):305–316. doi: 10.1084/jem.185.2.305

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Gene transcripts for GAPDH, β2-microglobulin, MHC class I heavy chain, and TAP1/TAP2 amplified from individual neurons and activated T cells. Cytoplasmic mRNA samples of individual neurons (lanes 1–5) and of individual T lymphoblasts (lanes 6 and 7) collected from single cells using the patch–clamp micropipette, reversely transcribed and coamplified by multiplex PCR with specific primers for GAPDH, β2microglobulin and MHC class I heavy chain (A). Whole brain tissue cDNA (lane B) and whole thymus tissue cDNA (lane T) as positive controls. Parallel coamplification of the same cDNA samples to detect TAP1 (B) and TAP2 (C) gene transcripts. Neurons (lanes 1–4), T lymphoblasts (lanes 5 and 6) or whole brain (lane B) and thymus tissue (lane T). Lane N and lane M show negative controls of PCR amplification and molecular weight marker X174/Hae III, respectively.